It was Salvador Oria who could not continue after the first set in his game against Jaime 'Chipi' Muñoz and Nacho Vilariño, forcing an early end to the encounter.
Eight-seed Virginia Riera and Tamara Icardo were back on the court after missing out in Chile, doing so with great success against veterans Eli Amatriain and Ana Catarina Nogueira.
La Rioja's shock finalists Valentino Libaak and Leandro Augsburger returned to action as well in Paraguay, drafted in as one of the five Lucky Losers.
Watch all of the highlights from centre court in the video below!
Matches in chronological order:
Claudia Fernandez (ESP)/Julieta Bidahorria (ARG) vs Bea Caldera (ESP)/Carmen Goenaga (ESP)
Marta Talavan (ESP)/Lorena Rufo (ESP) vs Jimena Velasco (ESP)/Sara Pujals (ESP)
Virginia Riera (ARG)/Tamara Icardo (ESP) (8) vs Eli Amatriain (ESP)/Ana C. Nogueira (POR)
Javi Barahona (ESP)/Javi Garcia Mora (ESP) vs 'Tonet' Sans (ESP)/Teo Zapata (ESP)
Jaime 'Chipi' Muñoz (ESP)/Nacho Vilariño (ESP) vs 'Josete' Rico (ESP)/Salva Oria (ESP)
Valentino Libaak (ARG)/Leandro Augsburger (ARG) vs Daniel Bressel (ESP)/Pablo Castillo (ESP)